Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus
Identifieur interne : 000116 ( Main/Exploration ); précédent : 000115; suivant : 000117Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus
Auteurs : Simon Thierry [France] ; Dongying Wang [France, République populaire de Chine] ; Pascal Arné [France] ; Manjula Deville [France] ; Barbara De Bruin [France] ; Adélaïde Nieguitsila [France] ; Christine Pourcel [France] ; Karine Laroucau [France] ; René Chermette [France] ; Weiyi Huang [République populaire de Chine] ; Françoise Botterel [France] ; Jacques Guillot [France]Source :
- BMC Microbiology [ 1471-2180 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Animaux (MeSH), Aspergillose (microbiologie), Aspergillose (médecine vétérinaire), Aspergillus fumigatus (classification), Aspergillus fumigatus (génétique), Aspergillus fumigatus (isolement et purification), Canards (MeSH), Dindons (MeSH), Données de séquences moléculaires (MeSH), Humains (MeSH), Maladies des oiseaux (microbiologie), Phylogenèse (MeSH), Répétitions minisatellites (MeSH), Techniques de typage bactérien (méthodes), Techniques génétiques (MeSH).
- MESH :
- génétique : ADN fongique, Aspergillus fumigatus.
- isolement et purification : Aspergillus fumigatus.
- microbiologie : Aspergillose, Maladies des oiseaux.
- médecine vétérinaire : Aspergillose.
- méthodes : Techniques de typage bactérien.
- Animaux, Canards, Dindons, Données de séquences moléculaires, Humains, Phylogenèse, Répétitions minisatellites, Techniques génétiques.
English descriptors
- KwdEn :
- Animals (MeSH), Aspergillosis (microbiology), Aspergillosis (veterinary), Aspergillus fumigatus (classification), Aspergillus fumigatus (genetics), Aspergillus fumigatus (isolation & purification), Bacterial Typing Techniques (methods), Bird Diseases (microbiology), DNA, Fungal (genetics), Ducks (MeSH), Genetic Techniques (MeSH), Humans (MeSH), Minisatellite Repeats (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Turkeys (MeSH).
- MESH :
- chemical , genetics : DNA, Fungal.
- classification : Aspergillus fumigatus.
- genetics : Aspergillus fumigatus.
- isolation & purification : Aspergillus fumigatus.
- methods : Bacterial Typing Techniques.
- microbiology : Aspergillosis, Bird Diseases.
- veterinary : Aspergillosis.
- Animals, Ducks, Genetic Techniques, Humans, Minisatellite Repeats, Molecular Sequence Data, Phylogeny, Turkeys.
Abstract
Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recently demonstrated for the avian major pathogen
We selected 10 VNTR markers located on 4 different chromosomes (1, 5, 6 and 8) of
MLVA displayed excellent discriminatory power. The method showed a good reproducibility. MST analysis revealed an interesting clustering with a clear separation between isolates according to their geographic origin rather than their respective hosts.
Url:
DOI: 10.1186/1471-2180-10-315
PubMed: 21143842
PubMed Central: 3004892
Affiliations:
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Le document en format XML
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Chine</country><wicri:regionArea>Parasitology department, College of Animal Science and Technology, Guangxi University, Nanning</wicri:regionArea></affiliation></author><author><name sortKey="Botterel, Francoise" sort="Botterel, Francoise" uniqKey="Botterel F" first="Françoise" last="Botterel">Françoise Botterel</name><affiliation wicri:level="1"><nlm:aff id="I6">UPE, UMR BIPAR, Ecopham, Faculté de Médecine de Créteil, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>UPE, UMR BIPAR, Ecopham, Faculté de Médecine de Créteil</wicri:regionArea></affiliation></author><author><name sortKey="Guillot, Jacques" sort="Guillot, Jacques" uniqKey="Guillot J" first="Jacques" last="Guillot">Jacques Guillot</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, 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xml:lang="fr">France</country><wicri:regionArea>ANSES, UMR BIPAR, Ecopham, Agence Nationale de Sécurité Sanitaire, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Dongying" sort="Wang, Dongying" uniqKey="Wang D" first="Dongying" last="Wang">Dongying Wang</name><affiliation wicri:level="1"><nlm:aff id="I1">ANSES, UMR BIPAR, Ecopham, Agence Nationale de Sécurité Sanitaire, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ANSES, UMR BIPAR, Ecopham, Agence Nationale de Sécurité Sanitaire, Maisons-Alfort</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Parasitology department, College of Animal Science and Technology, Guangxi University, Nanning, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Parasitology department, College of Animal Science and Technology, Guangxi University, Nanning</wicri:regionArea></affiliation></author><author><name sortKey="Arne, Pascal" sort="Arne, Pascal" uniqKey="Arne P" first="Pascal" last="Arné">Pascal Arné</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Deville, Manjula" sort="Deville, Manjula" uniqKey="Deville M" first="Manjula" last="Deville">Manjula Deville</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="De Bruin, Barbara" sort="De Bruin, Barbara" uniqKey="De Bruin B" first="Barbara" last="De Bruin">Barbara De Bruin</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Nieguitsila, Adelaide" sort="Nieguitsila, Adelaide" uniqKey="Nieguitsila A" first="Adélaïde" last="Nieguitsila">Adélaïde Nieguitsila</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Pourcel, Christine" sort="Pourcel, Christine" uniqKey="Pourcel C" first="Christine" last="Pourcel">Christine Pourcel</name><affiliation wicri:level="1"><nlm:aff id="I4">Université Paris Sud 11, CNRS, UMR 8621, Institut de Génétique et Microbiologie, Orsay 91405, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Université Paris Sud 11, CNRS, UMR 8621, Institut de Génétique et Microbiologie, Orsay 91405</wicri:regionArea></affiliation></author><author><name sortKey="Laroucau, Karine" sort="Laroucau, Karine" uniqKey="Laroucau K" first="Karine" last="Laroucau">Karine Laroucau</name><affiliation wicri:level="1"><nlm:aff id="I5">ANSES, Bacterial Zoonoses Unit, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Bacterial Zoonoses Unit, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Chermette, Rene" sort="Chermette, Rene" uniqKey="Chermette R" first="René" last="Chermette">René Chermette</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author><author><name sortKey="Huang, Weiyi" sort="Huang, Weiyi" uniqKey="Huang W" first="Weiyi" last="Huang">Weiyi Huang</name><affiliation wicri:level="1"><nlm:aff id="I2">Parasitology department, College of Animal Science and Technology, Guangxi University, Nanning, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Parasitology department, College of Animal Science and Technology, Guangxi University, Nanning</wicri:regionArea></affiliation></author><author><name sortKey="Botterel, Francoise" sort="Botterel, Francoise" uniqKey="Botterel F" first="Françoise" last="Botterel">Françoise Botterel</name><affiliation wicri:level="1"><nlm:aff id="I6">UPE, UMR BIPAR, Ecopham, Faculté de Médecine de Créteil, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>UPE, UMR BIPAR, Ecopham, Faculté de Médecine de Créteil</wicri:regionArea></affiliation></author><author><name sortKey="Guillot, Jacques" sort="Guillot, Jacques" uniqKey="Guillot J" first="Jacques" last="Guillot">Jacques Guillot</name><affiliation wicri:level="1"><nlm:aff id="I3">ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>ENVA, UMR BIPAR, Ecopham, Ecole nationale vétérinaire d'Alfort, Maisons-Alfort</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Aspergillosis (microbiology)</term><term>Aspergillosis (veterinary)</term><term>Aspergillus fumigatus (classification)</term><term>Aspergillus fumigatus (genetics)</term><term>Aspergillus fumigatus (isolation & purification)</term><term>Bacterial Typing Techniques (methods)</term><term>Bird Diseases (microbiology)</term><term>DNA, Fungal (genetics)</term><term>Ducks (MeSH)</term><term>Genetic Techniques (MeSH)</term><term>Humans (MeSH)</term><term>Minisatellite Repeats (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Turkeys (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Animaux (MeSH)</term><term>Aspergillose (microbiologie)</term><term>Aspergillose (médecine vétérinaire)</term><term>Aspergillus fumigatus (classification)</term><term>Aspergillus fumigatus (génétique)</term><term>Aspergillus fumigatus (isolement et purification)</term><term>Canards (MeSH)</term><term>Dindons (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Humains (MeSH)</term><term>Maladies des oiseaux (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Répétitions minisatellites (MeSH)</term><term>Techniques de typage bactérien (méthodes)</term><term>Techniques génétiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Aspergillus fumigatus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Bacterial Typing Techniques</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Aspergillose</term><term>Maladies des oiseaux</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Aspergillosis</term><term>Bird Diseases</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Aspergillose</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques de typage bactérien</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Aspergillosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Ducks</term><term>Genetic Techniques</term><term>Humans</term><term>Minisatellite Repeats</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Turkeys</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Canards</term><term>Dindons</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Phylogenèse</term><term>Répétitions minisatellites</term><term>Techniques génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recently demonstrated for the avian major pathogen <italic>Chlamydophila psittaci</italic>. In the present study, we developed a similar method for another pathogen of birds: the filamentous fungus <italic>Aspergillus fumigatus</italic>.</p></sec><sec><title>Results</title><p>We selected 10 VNTR markers located on 4 different chromosomes (1, 5, 6 and 8) of <italic>A. fumigatus</italic>. These markers were tested with 57 unrelated isolates from different hosts or their environment (53 isolates from avian species in France, China or Morocco, 3 isolates from humans collected at CHU Henri Mondor hospital in France and the reference strain CBS 144.89). The Simpson index for individual markers ranged from 0.5771 to 0.8530. A combined loci index calculated with all the markers yielded an index of 0.9994. In a second step, the panel of 10 markers was used in different epidemiological situations and tested on 277 isolates, including 62 isolates from birds in Guangxi province in China, 95 isolates collected in two duck farms in France and 120 environmental isolates from a turkey hatchery in France. A database was created with the results of the present study <ext-link ext-link-type="uri" xlink:href="http://minisatellites.u-psud.fr/MLVAnet/">http://minisatellites.u-psud.fr/MLVAnet/</ext-link>. Three major clusters of isolates were defined by using the graphing algorithm termed Minimum Spanning Tree (MST). The first cluster comprised most of the avian isolates collected in the two duck farms in France, the second cluster comprised most of the avian isolates collected in poultry farms in China and the third one comprised most of the isolates collected in the turkey hatchery in France.</p></sec><sec><title>Conclusions</title><p>MLVA displayed excellent discriminatory power. The method showed a good reproducibility. MST analysis revealed an interesting clustering with a clear separation between isolates according to their geographic origin rather than their respective hosts.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Arca Ruibal, B" uniqKey="Arca Ruibal B">B Arca-Ruibal</name></author><author><name sortKey="Wernery, U" uniqKey="Wernery U">U Wernery</name></author><author><name sortKey="Zachariah, R" uniqKey="Zachariah R">R Zachariah</name></author><author><name sortKey="Bailey, Ta" uniqKey="Bailey T">TA Bailey</name></author><author><name sortKey="Di Somma, A" uniqKey="Di Somma A">A Di Somma</name></author><author><name sortKey="Silvanose, C" uniqKey="Silvanose C">C Silvanose</name></author><author><name sortKey="Mckinney, P" uniqKey="Mckinney P">P McKinney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ghori, Hm" uniqKey="Ghori H">HM Ghori</name></author><author><name sortKey="Edgar, Sa" 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L" uniqKey="Alcazar Fuoli L">L Alcazar-Fuoli</name></author><author><name sortKey="Mellado, E" uniqKey="Mellado E">E Mellado</name></author><author><name sortKey="Klaassen, Ch" uniqKey="Klaassen C">CH Klaassen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duarte Escalante, E" uniqKey="Duarte Escalante E">E Duarte-Escalante</name></author><author><name sortKey="Zuniga, G" uniqKey="Zuniga G">G Zuniga</name></author><author><name sortKey="Ramirez, On" uniqKey="Ramirez O">ON Ramirez</name></author><author><name sortKey="Cordoba, S" uniqKey="Cordoba S">S Cordoba</name></author><author><name sortKey="Refojo, N" uniqKey="Refojo N">N Refojo</name></author><author><name sortKey="Arenas, R" uniqKey="Arenas R">R Arenas</name></author><author><name sortKey="Delhaes, L" uniqKey="Delhaes L">L Delhaes</name></author><author><name sortKey="Reyes Montes Mdel, R" uniqKey="Reyes Montes Mdel R">R Reyes-Montes Mdel</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>France</li><li>République populaire de Chine</li></country></list><tree><country name="France"><noRegion><name sortKey="Thierry, Simon" sort="Thierry, Simon" uniqKey="Thierry S" first="Simon" last="Thierry">Simon Thierry</name></noRegion><name sortKey="Arne, Pascal" sort="Arne, Pascal" uniqKey="Arne P" first="Pascal" last="Arné">Pascal Arné</name><name sortKey="Botterel, Francoise" sort="Botterel, Francoise" uniqKey="Botterel F" first="Françoise" last="Botterel">Françoise Botterel</name><name sortKey="Chermette, Rene" sort="Chermette, Rene" uniqKey="Chermette R" first="René" last="Chermette">René Chermette</name><name sortKey="De Bruin, Barbara" sort="De Bruin, Barbara" uniqKey="De Bruin B" first="Barbara" last="De Bruin">Barbara De Bruin</name><name sortKey="Deville, Manjula" sort="Deville, Manjula" uniqKey="Deville M" first="Manjula" last="Deville">Manjula Deville</name><name sortKey="Guillot, Jacques" sort="Guillot, Jacques" uniqKey="Guillot J" first="Jacques" last="Guillot">Jacques Guillot</name><name sortKey="Laroucau, Karine" sort="Laroucau, Karine" uniqKey="Laroucau K" first="Karine" last="Laroucau">Karine Laroucau</name><name sortKey="Nieguitsila, Adelaide" sort="Nieguitsila, Adelaide" uniqKey="Nieguitsila A" first="Adélaïde" last="Nieguitsila">Adélaïde Nieguitsila</name><name sortKey="Pourcel, Christine" sort="Pourcel, Christine" uniqKey="Pourcel C" first="Christine" last="Pourcel">Christine Pourcel</name><name sortKey="Wang, Dongying" sort="Wang, Dongying" uniqKey="Wang D" first="Dongying" last="Wang">Dongying Wang</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Dongying" sort="Wang, Dongying" uniqKey="Wang D" first="Dongying" last="Wang">Dongying Wang</name></noRegion><name sortKey="Huang, Weiyi" sort="Huang, Weiyi" uniqKey="Huang W" first="Weiyi" last="Huang">Weiyi 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